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Alternatively, the stock lifestyle organisms can be developed in an acceptable liquid medium (i.e., Soybeanone hundred fifty;Casein Digest Broth or Sabouraud Dextrose Broth) as well as cells harvested by centrifugation, then washed and resuspended in sterile saline TS to acquire a microbial count of about one × 108 cfu for every mL. [Be aware